bblkargonaut @ bblkargonaut @lemmy.world

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2 yr. ago

I will always have a special place in my heart for the Kevin timeline because it was my gateway drug to the good stuff. I wound up watching all of Star Trek in chronical order enterprise to Voyager.

Inspired by ohio valley style pizza

Pittsburgh has some of the worst drivers I've ever seen. Running red lights and waiting for yield signs to turn green is the norm there. I've seen at least 5 accidents caused by a Pittsburgher stopping at the end of an entrance ramp to a highway.

Unfortunately my car was finally sent to the body shop after 7 years dodging people, and 3 months from leaving the city by a lady who decided to make a left turn from the right lane. Luckily I always have a dashcam running, no one was hurt and the police wrote her a ticket. This lady was a high level Karen, and went ballistic when I asked if she was ok after she crashed into me. Best part was when her husband showed up 10mins later and apologized to me for his, "dumbass wife" who just knocked the mirrors off his truck.

I like to think that I'm a better critical thinker than most, but I fell for the initial news story about her being trans or intersex and the fight being unfair. Then I saw the pictures of her over the years and as a kid, and I dug deeper into what actually happened and I honestly feel dirty. I've since been unsubbing to a lot YouTubers.

Loll, his sacrifice was not in vain. I've still haven't seen the extended endings because I was satisfied with the original.

As a comic nerd I felt like I had brain damage after seeing origins in theaters. However, I do agree the major problem with the movie is the last act with "Deadpool" and the battle history was awesome. The video game based on the movie is awesome.

Mass effect for video games, Rebuild of Evangelion for movies, and Old Man war for Books. I know only mass effect is a trilogy (Andromeda doesn't exist) but I feel like all three stayed with me after finishing them, and the character development and plot lines all had satisfying conclusions.

If you live near a college, check their surplus or recycling. I got a brother printer for the low price of fixing a paper jam.

Come visit rural Minnesota

Minnesota is as much as a toss up as Alabama

I've been buying used 12tb HGST from Amazon for $80.

Wait until they learn the Spanish word for black, or worse Latin.

Someone in my group chat poster the picture and I can't unsee it. It's horrible.

My alignment is neutral good

Im the one that was paying for Netflix for my family, but the password crack down motivated me to learn how to build a server and go full arrs. They had a good thing going, but now that $26 a month will be used to buy hdds.

I just wanted to see Luke in the role of obi wan or Yoda after decades of Jedi training be a hero and pass on his legacy by training the next generation. But I would have been ok with one heroic lightsaber battle, and a reunion with han, chewy and Leia.

Watching the last Jedi and seeing someone who tossed his blade away because he saw the good in essentially "Space Hitler" try to kill his own nephew because he was having a nightmare so out of character. Then having him overdose on the force and die like a chump, broke me. I left the theater in silence. The last Jedi is also the only star wars movie with out a light saber fight. No blades ever touched.

A partial solution is to remove nimby laws that prevent accessory dwelling units, duplexes, and medium and high density housing. Then you use the tax laws to heavily punish corporate ownership of low and medium density housing, and make it progressively more expensive for mom and pop landlords after a reasonable number of properties.

Hopefully this would lower the cost for single family housing, while increasing supply and variety of rentals. But somehow you'd have to prevent end stage capitalist collusion from fixing prices so competition could actually work to drive down prices.

Lol yeh but it's partially funded by money saved from canceled subscriptions.

I'm a noob, I started 2 months ago with immich and tailscale. Now I have an unraid server, it's a slippery slope.

Pretty good description. Few extra points. -you don't need to don't need to do PCR to have enough Nucleic acids to visualize on the gel. Often you use gels to visualize things like purified plasmids. You can also digest your plasmids with enzymes to confirm genomic insert. -these type of gels have many uses outside of direct comparisons, for example you can use the gel purity DNA of a particular size by cutting out the band, melting the gel and using a silica column to pull the DNA out of the melted gel slice. This is super common when you do molecular cloning.

For what is possibly wrong with the top gel.

• the wavy line in some bands indicates uneven current, this can be due to sample composition before loading the gel, like having cellular debris along with your DNA or a mismatch between the buffer used to make the gel and the running buffer.

-the blurred bands can be a lot of things. It might just be inherited to the sample type, for example raw genomic DNA will look like this because you'll have enzyme that degrade DNA, and a lot of mid replication chromosomes resulting in fragments existing across a spectrum of sizes equally. Additionally the it could also be user error with overloaded well or not enough loading buffer in the sample so it's not pushed in to gel evenly, or it was run to fast.

Overall the top gel just looks like crap and the bottom gel looks great, but depending on what was run you really don't know what the gel should look like, if I was testing endonuclease activity the top gel would probably be what I was hoping to get. If was doing PCR on single gene, the bottom one is better.